[Pubmed] Feasibility of establishing a nude mouse model of thymoma-associated myasthenia gravis

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J Thorac Dis. 2025 Nov 30;17(11):10163-10171. doi: 10.21037/jtd-2025-1125. Epub 2025 Nov 25.

ABSTRACT

BACKGROUND: The pathogenesis of thymoma-associated myasthenia gravis (TAMG) remains unclear, and one of the key problems is the lack of an appropriate animal model. The present study aimed to investigate the feasibility of establishing an animal model of TAMG.

METHODS: Patient thymoma tissue specimens were transplanted into the left renal subcapsules of BALB/c nude mice under sterile conditions. The content of T lymphocytes and subsets, as well as total complement, in the mouse peripheral blood was detected using flow cytometry and enzyme-linked immunosorbent assay (ELISA) prior to and following transplantation. Furthermore, the tumor tissues were analyzed by immunohistochemistry (IHC).

RESULTS: Transplanted thymoma tissues survived in the mice with a success rate of 61%. Flow cytometry results indicated that the percentage of cluster of differentiation CD3+ and CD4+ T cells in the peripheral blood post-transplantation was significantly higher compared with pre-transplantation. By contrast, the percentage of CD8+ T cells and total complement cells was significantly reduced post-transplantation compared with pre-transplantation (P

Source: https://pubmed.ncbi.nlm.nih.gov/4137697 ... 22+67771e2